Taq DNA Polymerase Kit, 1000U

Taq for General PCR applications
 

Ultrapure, thermostable recombinant enzyme purified from the cloned Thermus aquaticus DNA Polymerase gene expressed in E.coli Composition of all optimized reagents for PCR reaction (Taq polymerase, Taq buffer, dNTP mix, loading dye and enhancer) in a kit One unit is the amount of enzyme required to catalyze the incorporation of 10nmoles of nucleotides into acid-insoluble material in 30 minutes at 74� A supply of enhancer, Magic buffer for GC rich template Flexible choice either Taq with reaction buffer containing MgCl2or Taq with MgCl2 free reaction buffer and separate MgCl2 Concentration: 5 units/ul 5'->3' exonuclease activity Amplification efficiency: ≥10 fold No Nuclease, RNase, Protease contamination Amplification range: lambda DNA : up to 6Kb (Max. 12Kb) human genomic DNA : up to 2Kb (Max. 4Kb) Stable at -20� Applications Amplification of genomic DNA and cDAN targets up to 3Kb long GC-rich sequences or secondary structures TA Cloning RT-PCR Different Display Mulitplex PCR Degenerate PCR PCR-based DNA fingerprinting (VNTR, STR and RAPD) etc.

Label

Content & Use

Taq DNA Polymerase

[5U/ul] in storage buffer 20mM Tris-Cl (pH 8.0),

100mM KCl, 0.1mM EDTA, 1mM DTT

0.5% Tween 20, 0.5% Nonidet P-40, 50% Glycerol

10X Taq buffer I (w/MgCl2)

100mM Tris-HCl (pH 8.3), 500mM KCl, 20mM MgCl2

10X Taq buffer II (w/o MgCl2)

100mM Tris-HCl (pH 8.3), 500mM KCl

MgCl2 Stock Solution

MgCl2

10X dNTP mix

2.5mM(each) dATP, dTTP, dGTP, dCTP

4X Magic Buffer

For amplification of GC rich template

10X Loading Dye

For gel loading

dH2O

DNase free water

Enzyme activity

PCR for 1ng of lambda(A) and E.coli(B) was performed using dilution series

of 5 different Taq DNA Polymerase with 1X, 1/5, 1/10, 1/50, and 1/100.

The Magic buffer is very useful to amplify high GC contents or complicated

structure of DNA. Human genomic DNA was amplified from 10ng to 3Kb by
adding Magic buffer (0.5~2x) to 1U of Taq DNA Polymerase.

No DNA contamination is shown in lane 4.

Cat. No.

Product

Size

K0561001

Taq DNA Polymerase

250U

K0561002

Taq DNA Polymerase

500U

K0561003

Taq DNA Polymerase

1000U

K0561004

Taq DNA Polymerase (MgCl2 Free Reaction Buffer)

250U

K0561005

Taq DNA Polymerase (MgCl2 Free Reaction Buffer)

500U

K0561006

Taq DNA Polymerase (MgCl2 Free Reaction Buffer)

1000U