EzWay�Direct PCR Buffer Set (5x)

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Komabiotech
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Phone:
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Contact name:
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PCR Taq Polymerase

	EzWay�Direct PCR Buffer Set (5x)

Click to enlarge image

Model Number

K0568002

for Direct PCR from various kinds of samples without a DNA extraction step

Using EzWay�Direct PCR buffer, DNA amplification is achieved from whole blood with a variety

of thermostable DNA polymerases. Amplification occurrs regardless of target size (up to 1.7 kb),

presence of various known PCR inhibitors, and high target GC content. Importantly, low copy

number DNA targets are effectively amplified from whole blood.

EzWay�Direct PCR buffer allows direct PCR from whole blood and may facilitate detection of

genetic diseases or infections by eliminating the time and effort for DNA extraction.

The use of EzWay Direct PCR buffer could facilitate the development of high-throughput PCR

for large-scale diagnostic screening or investigation of various medical conditions.

There is no need to purify DNA prior to PCR.
- It saves the time & cost to perform DNA purification.
- It gives convenience by omitting the complicated DNA purification process.
- It minimizes the risk of DNA contamination during reagent handling.
- It prevents the loss of trace samples in the DNA purification process.
- Easy PCR Handling and Automation
- Small amount of samples is feasible

DNA from various samples can be amplified directly without purification from the sample.
- Whole Blood, Blood Stains, Blood Cards, Buccal Swab, Saliva, Hair root, sperm, Body
  Fluid, Cultured Cells, Bacteria, Viruses, and Tissues (fresh, frozen or paraffinized) etc.

Any DNA polymerase can be used with EzWay�Direct PCR buffer for Direct PCR.
- Compatible with various sources of thermostable DNA polymerases (e.g. Taq, Pfu,
  Pwo, Tth DNA polymerase, all types of hot start DNA polymerase)

Hot start PCR effect without additional manual Hot Start Method or Hot start enzyme

5X EzWay Direct PCR Buffer, 500ul
5X Elution Buffer, 1.5ml
5X Lysis Buffer I, 1.5ml
Lysis Buffer II, 1.5ml

Viral infection, Molecular diagnostic test
Single cell diagnostic
Blood banking, Identity testing
Multiplex PCR. SNP detection, PCR-RFLP
Sequencing, Cloning
Laboratory automatic PCR

Sample	Direct Method	Lysis Method
Whole blood / serum / plasma	Directly add sample in anticoagulant vacutainer to 50 ul PCR reaction mixture.	1) Add lysis solution. 2) Incubate the tubes until completed lysis is achieved. 3) Spin down briefly. 4) Directly use 1 ul supernatant of crude lysate per 50 ul PCR reaction. * Some samples requires previous preparations such as centrifugation & collection of the supernatant before 1).
Cultured cells	Suspend cells from a plate and/or wash them with PBS, and directly add 1ul of cell suspension per 50 ul PCR reaction.
Buccal Cells	Immerse the brush into 10 ml PBS, and directly use 1 ul solution per 50 ul PCR reaction.
Saliva	Add 0.25 - 10 ul of saliva to 50 ul PCR reaction mixture.
Mouse tail	N/A
Paraffinized Tissue slide/TMA	After deparaffinization, scratch up the tissue of interest with syringe needle. Immerse the edge of the needle covered with tissue powders to 50 ul PCR reaction mixture and gently shake.
Tisseu/Organ/Meat	N/A
Hair root	N/A
Bacteria(Gram-) / Virus from feces	Suspend sample in PBS and crush well, centrifuge at 3,000 g for 1min and collect the supernatant, and directly use 1 - 10 ul solution per 50 ul PCR reaction.
Sputum	After liquefied, centrifuge and resuspend pellet in 50 ul of 1x Elution Buffer. Directly use 1 ul solution per 50 ul PCR reaction.
Bronchial lavage fluid/Urine	Centrifuge and resuspend the sample in 50 ul of 1x Elution Buffer. Directly use 1 ul solution per 50 ul PCR reaction.
Bacteria	For some gram negative bacteria such as E.coli, directly add one colony to 50ul PCR reaction with sterilized toothpick.
Plant/Fungus/Yeast	N/A
Dried blood, buccal cells on the papers	Punch a disk out of the sample area on the FTA Card, and then place 1-2 disk spot in a PCR tube and add PCR master mix directly to the disk and amplify.	N/A

■ Blood-direct PCR with various company's Taq polymerase

PCR amplifications were performed using various company's Taq DNA polymerase.

Taq DNA Polymerase	PCR Reaction Buffer
A : KOMA Taq DNA polymerase B : Taq DNA polymerase from competitor A C : Taq DNA polymerase from competitor B D : Taq DNA polymerase from competitor C E : Taq DNA polymerase from competitor D	Lane 1 : PCR Reaction Buffer f............ffrom each competitor Lane 2 : EzWay�Direct PCR Buffer Lane 3 : Blood Direct PCR Buffer ..............from a competitor

Cat. No.	Product	Size
K0568001	EzWay�Direct PCR Buffer (5x)	500ul
K0568002	EzWay�Direct PCR Buffer Set (5x) (Direct PCR Buffer, Elution Buffer, Lysis Buffer I & II)	1 set