EzWay�DNA Methylation Detection Express Kit, 50T

The Advanced method for detection of DNA methylation

The detection of methylation in CpG island of genomic DNA is one of the most important fields in

epigenetic studies. Koma introduces a new EzWay�DNA methylation detection kit that converts

unmethylated cytosine into uracil and leaves methylated cytosine as it is by Na-bisulfite.

EzWay�DNA methylation detection kit is ready to use kit including DNA extraction kit, DNA binding

and elution buffer, Na-bisulfite. The advantages of this kit lies in very high recovery rate that comes

from the proprietary binding and elution buffer for Na-bisulfite treated DNA.

This advanced EzWay�DNA methylation Detection Express Kit converts unmethylated cytosine into
uracil and leaves methylated cytosine as it is by Na-bisulfite in only 4 hours.

• Bisulfite modification & spin column method
• Complete conversion of unmethylated cytosine into uracil
• A fast C/U conversion step using heat denaturation
• Just 3-4 hours for whole procedures
• High recovery rate of modified DNA sample
• More simple DNA purification & elution procedure
• Sufficient volume of DNA Binding Buffer is supplied
• DNA Purification Kit is included
• A control primer is supplied for confirmation of DNA modification
• Application
  • MSP (Methylation specific PCR)
  • Methylation detection using DNA chip
  • COBRA (combined bisulfite restriction assay)
  • Methylate and Bisulfite sequencing etc.