EzWay�Buccal Swab DNA Isolation Kit

Spectrophotometric data for the genomic DNA isolated from buccal swab samples is given in Table 1.

Genomic DNA was analyzed by agarose gel electrophoresis (Figure 1). Undigested DNA was resolved

on a 1% agarose gel and visualized by ethidium bromide staining. As shown in Figure 2, PCR

amplification of GAPDH gene using control primer was done successfully. Yields of DNA from swabs are

highly variable depending on the swab type, the person being swabbed, the swabbing technique,

and the number of cells captured on the swab. The expected yield using this protocol is 300 ng to 3 ug

per swab.

       Table 1. Genomic DNA isolation from buccal swabs. Cotton swabs were used on twelve different donors.
      Yields in this study were measured using a NanoDrop® ND-1000 instrument.

       Figure 1. Isolated genomic DNA

      4 ul of each isolated template from buccal swab was

      resolved on a 1% agarose gel and visualized by

      ethidium bromide staining.

Figure 2. PCR amplified of GAPDH gene

4 ul of each PCR product using control primer was

resolved on a 1.2% agarose gel and visualized by

ethidium bromide staining.